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Provided by the project REP-LECOTOX, INCO-CT-2006-043559-REP-LECOTOX



7900HT Fast Real - Time PCR System with TaqMan  Low Density Array Block

Will be used:

* To integrate molecular and traditional ecosystem measurements for understanding stressor impacts;

* To use  gene expression as biomarkers of environmental contaminants together with traditional function-based biomarkers, which may be used to validate the toxic mechanisms of the contaminant. 

* For investigation of mechanisms of endocrine disruption



The TaqMan Low Density Array is a micro fluidic card for quantitative real-time PCR. TaqMan arrays are delivered with TaqMan Gene Expression Assays pre-loaded into each of the 384 reaction wells according to the selected format. The TaqMan array is designed for use on the flexible Applied Biosystems 7900HT Fast Real-Time PCR System with a 7900HT TaqMan





Veriti Thermal Cycler, 96-Well


DBE_FSUNS possesses relatively good equipment, and all equipment is available to the researchers of LECOTOX.
 Existing equipement (in function):


Equipement for PCR   Termocycler (24 places)

Multiscan Plate reader (Thermo–Labsystems)  - 8 filters, spectral range: 400-750 nm


Safety bench (HPH 12 Heraguard–Kendro )and CO2 incubator (POLY TEMP SCI) for cell cultures

Fluoroskan Ascent Thermo–Labsystems for fluorimetric and luminometric measurements – with adapters for microtitar plates of different size, 8 excitation and 8 emmission filters for fluorimetric measurements

Ultracentrifuge L8-70M Beckman  

Wallac 1410-scintillation counter;

Equipment for Western blott-Novex,

Equipment for horizontal, vertical and rocket electrophoresis and dot-blot,

Eppendorf refrigerated centrifuge, Eppendorf bench-top centrifuge


 
Thursday, 09 September 2010